Part:BBa_K562011:Experience

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••• iGEM Dundee 2011

This part was seen to work in practice. The PduD40 tag fused to mCherry resulted in fluorescent cells (Figure 1).

Results

E. coli was transformed with BBa_K562011 and grown overnight in LB medium under aerobic conditions. A sample of whole cells was taken, washed in phosphate-buffered saline, and applied to a microscope slide using CellTak adhesive. The cells were then observed by confocal microscopy and shown to be fluorescent (Figure 1).

Figure 1: mCherry is produced and fluorescent when fused to the PduD40 signal sequence.

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